Pick leaders in your niche who have lots of followers. The quickest way to get Followers is to follow others. Statistically, 30-60% will follow you back as will some of their followers. You can have more than one twitter account which means you could be adding hundreds of new followers every day! You can build targeted online audience very quickly.
Look for a search box at the top of your Twitter page. Type in a key word describing your niche. Twitter will display possible choices and at the bottom of the drop down menu it will say “View all people.” Choose that. That will list all the people in that niche. Click on each persons name and it will display how many followers they have. My niche is Emergency Preparedness. I searched on that and found an organization in Australia with 26k followers. My niche is global so Australia is fine. If I follow 100 people a day that will be 30 followers a day. It will take me 9 months to go through that list. and I should end up with 8000 followers.
Most people who are going to follow you back will do so within a couple of days. So every 3 days Unfollow people who don't follow you back. Followers are interested in you. Non-Followers aren’t interested and can prevent you from optimizing your list. Your target is at least 5000 members.
The formula is: Current Followers - non-Followers + Fresh Followers = Your targeted list.